Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)
![Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)](https://www.abcam.com/ps/products/1/ab1285/Images/ab1285-4144-mouse-igg1monoclonal-hybigg1-fitc-phycoerythrin-isotype-control-immunocytochemistry-immunofluorescence.jpg "Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)")
Overview
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Product name
Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control
See all Mouse isotype controls -
Conjugation
Dual color: FITC + PE -
Tested applications
Suitable for: Flow Cytmore details -
General notes
This product is a mixture of the same clone separately bearing both FITC and PE labels. This product contains sodium azide, which under acid conditions yields hydrazoic acid, a toxic compound. Azide compounds should be diluted with running water before being discarded to avoid deposits in lead or copper plumbing where explosive conditions may develop.This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Isotype control notes
This antibody control duo is a direct immunofluorescence reagent used as an isotype (negative) control for flow cytometric immunophenotyping of erythrocyte-lysed whole blood with monoclonal antibodies. -
Clonality
Monoclonal -
Clone number
HybIgG1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
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Alternative names
- Mouse Isotype Control
Images
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Immunocytochemistry/ Immunofluorescence - Dual color: FITC + PE Mouse IgG1monoclonal [HybIgG1] - isotype control (ab1285)ICC/IF image of ab2185 stained MCF7 cells. The cells were 100% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2185, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
