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Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Overview

  • Product name

    Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control
    See all Mouse isotype controls

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, IHC-Frmore details

  • Immunogen

    Other Immunogen Type corresponding to Mouse. Synthetic hapten

  • General notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    ab91353 is purified and passed through a 0.22µm filter

  • Isotype control notes

    ab91353 enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue. Suitable for whole blood, Ficoll-separated preparations, frozen and paraffin embedded sections

  • Clonality

    Monoclonal

  • Clone number

    B11/6

  • Isotype

    IgG1

  • Light chain type

    kappa

  • Research areas

  • Alternative names

    • Mouse Isotype Control

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353) Karanovic et al One. 2016 Aug 25;11(8):e0161706. doi: 10.1371/journal.pone.0161706. eCollection 2016. Fig 9. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Glomerular nestin expression in experimental groups.

    (A) Negative control (×200).

    (C) SHADR group: Diffuse glomerular nestin expression was detected involving almost all podocytes within glomerulus. After losartan and tempol treatment, either single or in combination, kidneys restored nestin expression similar to controls (SHC group).

    Immunostaining was applied on 5 μm thick paraffin sections. After deparaffinization and rehydration, the sections were treated by microwave for 20 minutes at 400 W in citrate buffer (pH 6.0). After antigen retrieval, samples were incubated for 1 hour at room temperature with primary antibody for nestin (dilution 1:100). Sections were then treated using 3-amino-9-ethylcarbazole (AEC) as substrate, and counterstained with hematoxylin. Negative controls were performed by omitting the first antibody and mouse monoclonal antibodies as isotype control mouse IgG1 (ab91353) antibody was also used.

  • Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353) Hirakawa et al PLoS One. 2016 Aug 3;11(8):e0159912. doi: 10.1371/journal.pone.0159912. eCollection 2016. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Effects of hypoxia on mRNA expression of IGF1R and IGF1, and production of IGF1 in cancer cells.

    Cell surface expression of IGF1R by FACScan analysis. IGF1R expression level of Panc-1, RWP-1, OCUP-AT, and MiaPaCa-2 cells was higher in hypoxia than that in normoxia.

    Cells (2 x 106 cells/mL) were fixed with 2% paraformaldehyde and incubated in PBS with anti IGF1R antibody (ab16890, Abcam) or mouse IgG1- isotype control (ab91353, Abcam) for 30 minutes at 22°C. Cells were subsequently labeled with FITC-conjugated secondary antibody (1:500; ab96879, Abcam) for 30 minutes at 22°C.

    The percentage of positive cells were calculated and compared with isotype-matched control-stained cells.

  • Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    Flow Cytometry - Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Overlay histogram showing HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained with ab59509 (red line).

    The cells were fixed with 80% methanol (5 minutes) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59509, 1 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG, H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC.

    Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions.

    Acquisition of >5,000 events was performed.

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