Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)
![Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)](https://www.abcam.com/ps/products/18/ab18413/Images/ab18413-277417-mouse-igg2a-kappa-monoclonal-mopc-173-isotype-control-chip-grade-chip.jpg "Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413)")
Overview
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Product name
Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade
See all Mouse isotype controls -
Description
Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade -
Specificity
This Balb/c myeloma derived clone has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues. -
Tested applications
Suitable for: Flow Cyt, ChIP/Chip, ChIPmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MOPC-173 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
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Alternative names
- Mouse Isotype Control
Images
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ChIP - Mouse IgG2a, Kappa Monoclonal [MOPC-173] - Isotype Control - ChIP Grade (ab18413) Image from Niemantsverdriet M et al., PLoS One. 2012;7(12):e50815. Fig 5.; doi: 10.1371/journal.pone.0050815. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
ChIP of ΔNp73 binding with SBE. The relative amount of ΔNp73 associated DNA as pulled down with an antibody directed against HA, is represented as a fold enrichment compared to pull-down with IgG (background, ab18413). Gene enrichment was quantified by qPCR using primers specific for the promoter regions of A) PAI-1, B) Col1a1 and C) p21WAF within the SBEs. Primers specific for PTEN (D) were used as a positive control. Pulldown antibody is shown on the x-axis, with y-axis showing fold enrichment ± SEM. * represents p-value>0.05.