Antibodies, Proteins, Kits and Reagents for Life Science

303 424 ₸ Product size

100 µl 303 424 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RBP4 antibody [EPR18020-115] (ab188230)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR18020-115] to RBP4
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Reacts with: Mouse, Rat

Product name

Anti-RBP4 antibody [EPR18020-115]
See all RBP4 primary antibodies
Description

Rabbit monoclonal [EPR18020-115] to RBP4
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse
ICC/IF	Mouse
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Rat

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse kidney tissue lysate and serum; Rat kidney tissue lysate; Hepa1-6 whole cell lysate. IP: Hepa1-6 whole cell lysate. IHC-P: Mouse and rat liver tissues. ICC/IF: Hepa1-6 cells. Flow Cyt: Hepa1-6 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR18020-115
Isotype

IgG
Research areas

Western blot - Anti-RBP4 antibody [EPR18020-115] (ab188230)

All lanes : Anti-RBP4 antibody [EPR18020-115] (ab188230) at 1/1000 dilution

Lane 1 : Mouse serum
Lane 2 : Mouse kidney tissue lysate
Lane 3 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
Lane 4 : Rat kidney tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 23 kDa
Observed band size: 21 kDa
why is the actual band size different from the predicted?

Exposure times: Lanes 1, 2 and 4: 1 minute; Lane 3: 30 seconds.

Blocking/dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of mouse liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBP4 antibody [EPR18020-115] (ab188230)

Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling RBP4 with ab188230 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining on hepatocytes of rat liver (PMID: 19624771; PMID: 21324757) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-RBP4 antibody [EPR18020-115] (ab188230)

Immunofluorescent analysis of 4% paraformalehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling RBP4 with ab188230 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.
Flow Cytometry - Anti-RBP4 antibody [EPR18020-115] (ab188230)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling RBP4 with ab188230 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-RBP4 antibody [EPR18020-115] (ab188230)

RBP4 was immunoprecipitated from 0.35 mg of Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate with ab188230 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab188230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1: Hepa1-6 whole cell lysate 10 μg (Input).

Lane 2: ab188230 IP in Hepa1-6 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab188230 in Hepa1-6 whole cell lysate.

Exposure time: 2 seconds.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Anti-RBP4 antibody [EPR18020-115] (ab188230)

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