Canine PDGF-BB ELISA Kit (ab273171)
Key features and details
- Sensitivity: 4 pg/ml
- Range: 12.29 pg/ml - 1200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Dog
Overview
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Product name
Canine PDGF-BB ELISA Kit
See all PDGF B kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4 pg/ml -
Range
12.29 pg/ml - 1200 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 141.6 134% - 148% Serum 132.2 122% - 147% Plasma 130.2 121% - 148% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Dog -
Product overview
Canine PDGF-BB ELISA Kit (ab273171) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of canine PDGF-BB in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for canine PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti-Canine PDGF-BB coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Canine PDGF-BB Antibody 2 vials Canine PDGF-BB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
- Information by UniProt
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Alternative names
- Becaplermin
- c sis
- FLJ12858
see all -
Database links
Images
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Canine PDGF-BB ELISA Kit standard curve
This standard curve is for demonstration only. A standard curve must be run with each assay.