Uricase Assay Kit (Fluorometric) (ab234042)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Tissue Extracts
- Sensitivity: 10 µU
Overview
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Product name
Uricase Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue Extracts -
Assay type
Enzyme activity -
Sensitivity
10 µU -
Assay time
1h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Uricase Assay Kit (Fluorometric) (ab234042) provides a quick and easy method for the measurement of uricase activity in a wide variety of samples.
In this assay, uricase oxidizes uric acid forming a product that reacts with the probe, thus generating a fluorescence signal (Ex/Em= 535/587nm). The generated fluorescence signal is directly proportional to the level of uricase activity in the sample. The kit provides a sensitive, high throughput and adaptable assay that can measure uricase activity as low as 10 µU in biological samples.
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Notes
Uricase (factor-independent urate hydroxylase, urate oxidase (UO); EC 1.7.3.3) is an enzyme involved in the uric acid metabolism. Uric acid is the end product of purine metabolism, and high levels of uric acid in blood causes gout. Uricase is present in a wide range of mammals but absent from human beings.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests H2O2 Standard (0.88 M) 1 x 100µl Uricase Assay Buffer 1 x 50ml Uricase Enzyme Mix 1 vial Uricase Positive Control 1 vial Uricase Probe (in DMSO) 1 x 200µl Uricase Substrate 1 x 10ml -
Research areas
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Relevance
Uricase catalyzes the oxidation of uric acid to 5 hydroxyisourate which spontaneously decomposes to form allantoin. -
Cellular localization
Peroxisome. -
Alternative names
- Factor-independent urate hydroxylase
- UOX
- Urate oxidase
see all
Images
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Uricase assay example standard curve.
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Uricase activity in rat liver and spinach leavesUricase activity in rat liver (5.57 μg protein), spinach leaves (31.5 μg protein) and Uricase Positive Control.
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Uricase specific activity in rat liver and spinach leaves