Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (ab273287)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 4 µU
Overview
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Product name
Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
4 µU -
Assay duration
Multiple steps standard assay -
Product overview
Tyrosine Aminotransferase Activity Assay Kit (Fluorometric) (ab273287) is a simple plate-based fluorometric assay for the measurement of Tyrosine Aminotransferase (EC 2.6.1.5), also known as Tyrosine Transaminase (TAT) activity in biological samples. The transamination of tyrosine by TAT produces glutamate (through the transfer of amine group to α-ketoglutarate), which converts a non-fluorescent probe to a fluorescent product via an enzymatic reaction. The assay can detect as low as 4 µU of tyrosine aminotransferase.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Glutamate Standard (0.1 M) 1 x 0.1ml TAT Assay Buffer 1 x 25ml TAT Developer (Lyophilized) 1 vial TAT Enzyme Mix (Lyophilized) 1 vial TAT Positive Control (Lyophilized) 1 vial TAT Probe 1 x 0.4ml TAT Substrate I (Lyophilized) 1 vial TAT Substrate II (Lyophilized) 1 vial
Images
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Glutamate standard curve.
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Enzyme kineticsEnzyme kinetics for rat liver lysate (4.4 µg protein per well) and Jurkat cell lysate (10 µg protein per well).
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TAT activity in rat liver tissue lysate and Jurkat cell lysate.TAT activity in rat liver tissue lysate and Jurkat cell lysate.