Total Choline Assay Kit (ab219944)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 40 nM
Overview
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Product name
Total Choline Assay Kit -
Detection method
Fluorescent -
Sample type
Urine, Serum, Plasma, Other biological fluids, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
40 nM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Total Choline Detection Kit (ab219944) provides a sensitive fluorescence-based assay for quantifying choline in cell and tissue lysates and biological fluids. The amount of choline is proportional to the concentration of hydrogen peroxide formed in the choline oxidase-mediated enzyme coupling reaction cycle, which in turn is detected with our proprietary Red Indicator. The increase in fluorescence can be easily detected at an optimal Ex/Em = 540/590 nm. The signal can also be read by absorbance at OD: 576 ± 5 nm, although the sensitivity of the assay is reduced 10-fold.
The assay detects as little as 4 picomole choline in 100 µL assay volume (40 nM), making it one of the most sensitive assays available in the market.
This product was previously known as Total Choline Detection Kit.
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Notes
Choline is an essential nutrient. Choline and its metabolites play an important role in the structural integrity and signaling of cell membranes and cholinergic neurotransmission (choline synthesis). It is a major source of methyl group via its metabolite, trimethylglycine that participates in the S-adenosylmethionine synthesis pathways. Choline deficiency may cause liver disease, atherosclerosis and possibly neurological disorders. Despite its importance in the central nervous system as a precursor for acetylcholine and membrane phosphatidylcholine, the role of choline in mental illness has been little studied.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 25ml Choline Probe 2 vials Choline Standard (2.8 mg) 1 x 2.8mg DMSO 1 x 100µl Red Dye 1 vial
Images
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Total Choline Detection Kit (ab219944)
Typical Choline standard calibration curve. Choline dose response was measured on a 96 well solid black plate using a Gemini fluorescence microplate reader (Molecular Devices). As low as 40nM (4 picomole/well) of choline can be detected with 30 minutes incubation time (n=3).
