Tissue Factor Activity Assay Kit (Human, Colorimetric) (ab108906)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Plasma, Serum, Tissue, Urine
Overview
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Product name
Tissue Factor Activity Assay Kit (Human, Colorimetric)
See all Tissue Factor kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Cell culture extracts, Tissue -
Assay type
Enzyme activity (quantitative) -
Assay time
1h 00m -
Species reactivity
Reacts with: Human -
Product overview
Tissue Factor Activity Assay Kit (Human, Colorimetric) ab108906 is used to determine Human Tissue Factor activity in plasma, tissue and cell culture supernatants.
The tissue factor activity assay protocol measures the ability of lipoprotein TF/FVIIa to activate factor X (FX) to factor Xa. The amidolytic activity of the TF/FVIIa complex is quantitated by the amount of FXa produced using a highly specific FXa substrate releasing a yellow para-nitroaniline (pNA) chromophore. The change in absorbance of the pNA at 405 nm is directly proportional to the TF enzymatic activity.
Tissue factor activity assay protocol summary:
- add reaction mix to wells
- add standards and samples
- incubate for 30 min
- add FXa substrate
- analyze with a microplate reader every 5 min for 25 minThe entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.
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Notes
Previously called Tissue Factor Human Chromogenic Activity Assay Kit.
The transmembrane protein Tissue factor (TF) is the physiologic trigger of coagulation in normal hemostasis. Tissue factor binds and allosterically activates factor VII (FVII). The TF-FVIIa complex cleaves factor IX and X, leading to thrombin generation. Tissue factor markedly enhances the ability of FVIIa to cleave both macromolecule and small peptidyl substrates.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Diluent 1 x 20ml FXa Substrate (Lyophilized) 2 vials Human FVII (Lyophilized) 1 vial Human FX (Lyophilized) 1 vial rh Tissue Factor Standard (lipoprotein) (Lyophilized) 1 vial Sample Diluent 1 x 11ml Sealing Tapes 3 units Tissue Factor Microplate (12 strips of 8 wells) 1 unit -
Research areas
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Function
Initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex activates factors IX or X by specific limited protolysis. TF plays a role in normal hemostasis by initiating the cell-surface assembly and propagation of the coagulation protease cascade. -
Sequence similarities
Belongs to the tissue factor family. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CD142
- CD142 antigen
- Coagulation factor III
see all -
Database links
- Entrez Gene: 2152 Human
- Omim: 134390 Human
- SwissProt: P13726 Human
- Unigene: 62192 Human
Images
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Functional studies - Tissue Factor Human Chromogenic Activity Assay Kit
Colorimetric standard curve: mean of duplicates (+/- SD)
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Functional studies - Tissue Factor Human Chromogenic Activity Assay KitStandard curve showing concentration (pM) on X-axis and increase of O.D. per min on Y-axis (time frame: 20 min.)
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Functional studies - Tissue Factor Human Chromogenic Activity Assay KitTime course showing O.D.'s of one standard point and one sample (undiluted vs.diluted)
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Functional studies - Tissue Factor Human Chromogenic Activity Assay KitTissue Factor measured in various biologicals showing average concentrations
