Sulfatase Activity Assay Kit (Colorimetric) (ab204731)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Sulfatase Activity Assay Kit (Colorimetric)
See all Sulfatase kits -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Enzyme activity -
Product overview
Sulfatase Activity Assay Kit (ab204731) provides a quick and easy way to determine sulfatase activity. The kit measures the hydrolysis of a sulfate ester to 4-nitrocatechol, which can be detected at OD=515 nm. The kit is suitable for measuring activity of purified enzyme as well as sulfatase from biological samples. The limit of detection is below 1 mU.
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Notes
Sulfatases (EC 3.1.6) are enzymes in the esterase class that catalyze the hydrolysis of sulfate esters from a wide range of biological molecules, including steroids, carbohydrates, and proteins. They can be found in intracellular and extracellular spaces and are distributed in a wide range of cells and tissues. Intracellular sulfatases are commonly found localized within the lysosome. Genetic defects in sulfatase can result in certain lysosomal storage disorders and abnormal expression can contribute to certain hormone-dependent cancers, such as breast and prostate cancer.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests 4-Nitrocatechol Standard (0.5 mM) Yellow 1 x 1.5ml Stop Solution NM 1 x 10ml Sulfatase Green 1 vial Sulfatase Assay Buffer NM 1 x 5ml Sulfatase Substrate Amber/NM 1 x 4ml
Images
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Sulfatase Activity Assay Kit (ab204731)
Typical 4-Nitrocatechol Standard calibration curve.
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Sulfatase Activity Assay Kit (ab204731)Enzyme activity measuring different amounts of the provided Sulfatase.
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Sulfatase Activity Assay Kit (ab204731)Measurement of Sulfatase Activity in HepG2 cell lysate (30 µg) and rat liver homogenate (100 µg).