Ube2L6 overexpression 293T lysate (whole cell) (ab94307)
Overview
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Product name
Ube2L6 overexpression 293T lysate (whole cell) -
General notes
ab94307 is a 293T cell transfected lysate in which Human Ube2L6 has been transiently over-expressed using a pCMV-Ube2L6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Catalyzes the covalent attachment of ubiquitin or ISG15 to other proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Tissue specificity: Present in natural killer cells (at protein level). Pathway: Protein modification; protein ubiquitination. Similarity: Belongs to the ubiquitin-conjugating enzyme family. PTM: ISGylated.
Images
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SDS-PAGE - Ube2L6 overexpression 293T lysate (whole cell) (ab94307)ab94307 at 15μg/lane on an SDS-PAGE gel.
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Western blot - Ube2L6 overexpression 293T lysate (whole cell) (ab94307)All lanes : Anti-Ube2L6 antibody (ab56502) at 1/500 dilution
Lane 1 : Ube2L6 293T Transfected Lysate - Positive Control (ab94307)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution
