Streptavidin Resin - Amintra (ab270530) provides a simple, rapid and reliable method to purify biotin and biotinylated substances.

This product is manufactured by Expedeon, an Abcam company, and was previously called Amintra Streptavidin Resin. Expedeon product codes ASA0002 (2 mL Medium), ASA0005 (5 mL Medium) and ASA0010 (10 mL Medium) are the same as the current sizes: 2 mL, 5 mL, and 10 mL, respectively.

Purified streptavidin isolated from Streptomyces avidinii is immobilized on highly cross-linked 6% agarose. The immobilized streptavidin binds biotin and biotinylated substances and can be used for affinity chromatography applications. The interaction between streptavidin and biotin is very strong and requires denaturing conditions for elution, which may destroy both the ligand and the sample. Alternatively, it can be used in the purification of antigens, where biotinylated antibodies are incubated with antigen.

The biotinylated antibody-antigen complex binds to Streptavidin Resin - Amintra from which the antigen can be eluted. The base matrix of Streptavidin Resin - Amintra is a robust, high- cross linked 6% agarose. The crosslinking of the base matrix has been optimized to give the matrix good flow properties and high physical and chemical stability, both of which are key factors for cost-effective, large-scale use.

Additional Information:

• Ligand: Streptavidin
• Binding capacity: Biotin > 120 nmol/ml medium; Biotinylated serum albumin 6 mg/ml medium
• Particle size: 45 µm – 165 µm
• Bead structure: Highly cross-linked 6% agarose
• Maximum back pressure: 0.3 MPa (3 bar, 43 psi)
• Recommended linear flow rate*: 300 – 500 cm/h
• pH stability: 2 – 10
• Temperature stability: 2ºC to 8ºC
  • Short term: 2ºC to room temperature
  • Long term: 2ºC to 8ºC
• Storage buffer: 20% ethanol