Streptavidin Atto 542 Monovalent Antibody Labeling Kit (ab272188)
Overview
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Product name
Streptavidin Atto 542 Monovalent Antibody Labeling Kit -
Product overview
Streptavidin Atto 542 Monovalent Antibody Labeling Kit ab272188 is used to label biotinylated antibodies with Atto 542.
Atto 542 is very bright and can be used as an alternative to Cy3®.
The Antibody - Streptavidin Atto 542 complex produced with this kit is stable and can be used in the same way as antibodies conjugated directly with fluorochromes.
Several antibodies can be labeled with this kit, and its associated kits, and used together in the same experiment.
Use this kit to:
- label biotinylated antibodies with fluorescent dyes, and use them in immunostaining just like a directly dye conjugated antibody- label two or more biotinylated antibodies with different fluorescent dyes* and then use them together in the same experiment
*multiple kits required, see list below- label as little as 0.1 µg of biotinylated antibody in less than 5 mins without washes
Technical notes
The 50 µg size of this kit is typically sufficient to label 50 µg of antibody, the 25 µg size is sufficient to label 25 µg of antibody.When the antibody - streptavidin complex is produced with this kit, due to the monovalent nature of the streptavidin and the blocking solution, there is no risk of forming aggregates of multiple antibodies.
The antibody - streptavidin complex is also very stable, if 2 or more biotinylated antibodies are labeled with different dyes, and then mixed, no dye will transfer between the antibodies.
List of available monovalent streptavidin antibody labeling kits
Streptavidin Alexa Fluor® 488 (ab272187)
Streptavidin Atto 542 (ab272188) – this kit
Streptavidin Alexa Fluor® 594 (ab272189)
Streptavidin Alexa Fluor® 647 (ab272190)
Note: the streptavidin in this kit contains very small amounts of non-monovalent streptavidin, when used with the blocking buffer included in the kit, this does not affect the conjugated antibodies. -
Tested applications
Suitable for: Flow Cyt, IHCmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 50 µg 25 µg Biotin block buffer 1 x 500µl 1 x 250µl Streptavidin Atto 542 labeling reagent (Monovalent) 1 x 500µl 1 x 250µl
Images
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Multiplex 4 color staining
Multiplex immunostaining of a frozen tissue section of mouse spleen using four biotinylated antibodies labeled using Streptavidin (Monovalent) kits. T cells (rat anti-CD3, 7 µg/ml, green), B cells (rat anti-CD45R, 7 µg/ml, red), macrophages (rat anti-CD11b, 7 µg/ml, white), and dendritic cells (hamster anti-CD11c, 7 µg/ml, blue) were detected using Streptavidin (Monovalent) kits with four different fluorochromes. Picture was taken with a standard fluorescence microscope equipped with appropriate filters for optimal multicolor separation.
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Brightness compared to directly conjugated antibodiesAntibodies labeled with Streptavidin (Monovalent) kits are as bright as directly conjugated antibodies. Flow cytometry analysis of splenic B lymphocytes immunolabeled with antibodies against CD45R (B220, clone RA3-6B2, 7 µg/ml). Dark green = directly conjugated antibody, light green = Streptavidin (Monovalent)-labeled antibody.
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No spillover of signals from different labels