Stimulatory Immune Checkpoint Panel - Human IHC (ab278178)
Overview
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Product name
Stimulatory Immune Checkpoint Panel - Human IHC -
Species reactivity
Reacts with: Human -
Product overview
Stimulatory Immune Checkpoint Panel - Human IHC ab278178 contains multiple trial-sized versions of anti-human antibody clones against CD134 / OX40L, ICOS, CD40, CD28, CD27, CD226, TMEM173 and GITR specifically selected for high performance in various applications. They are provided as a sampler panel to allow you to easily evaluate each antibody.
For guidelines on how to use each antibody within the panel, please consult the individual datasheet for each antibody.
Panel contains:
- Rabbit monoclonal [EPR23000-42] to CD134 / OX40L receptor (20 μL) ab264466
- Rabbit monoclonal [EPR20560] to ICOS (20 μL) ab224644
- Rabbit monoclonal [EPR20735] to CD40 (20 μL) ab224639
- Rabbit monoclonal [EPR22076] to CD28 (20 μL) ab243228
- Rabbit monoclonal [EPR8569] to CD27 (20 μL) ab131254
- Rabbit monoclonal [EPR21172] to CD226 (20 μL) ab212076
- Rabbit monoclonal [EPR13130-55] to TMEM173 (20 μL) ab239074
- Rabbit monoclonal [CAL8] to GITR (20 μL) ab237714
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Notes
Explore our range of antibody sample panels designed to provide you with a variety of trial-size antibodies in a convenient and cost-effective format.
Directly conjugated versions of our antibodies are available and ready to use for multicolor flow cytometry or immunocytochemistry analysis. Please refer to the ‘Associated products’ section below.
Carrier-free formulations of our recombinant antibodies are also available for easy conjugation to labels of your choice and for multiplex applications. Please refer to the ‘Associated products’ section below.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit ab264466 - Anti-CD134 / OX40L receptor antibody [EPR23000-42] 2 x 10µl ab212076 - Anti-CD226 antibody [EPR21172] 2 x 10µl ab131254 - Anti-CD27 antibody [EPR8569] 2 x 10µl ab243228 - Anti-CD28 antibody [EPR22076] 2 x 10µl ab224639 - Anti-CD40 antibody [EPR20735] 2 x 10µl ab237714 - Anti-GITR antibody [CAL8] 2 x 10µl ab224644 - Anti-ICOS antibody [EPR20560] 2 x 10µl ab239074 - Anti-TMEM173 antibody [EPR13130-55] 2 x 10µl -
Research areas
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Cellular localization
CD40: Secreted and Cell membrane. CD28: Membrane. CD27: Membrane. ICOS: Secreted and Cell membrane. CD134 / OX40L receptor: Membrane. GITR: Secreted and Cell membrane. CD226: Membrane. TMEM173: Endoplasmic reticulum membrane. Mitochondrion outer membrane. Cell membrane. Cytoplasm > perinuclear region. In response to double-stranded DNA stimulation, relocalizes to perinuclear region, where the kinase TBK1 is recruited. -
Database links
- Entrez Gene: 10666 Human
- Entrez Gene: 29851 Human
- Entrez Gene: 340061 Human
- Entrez Gene: 7293 Human
- Entrez Gene: 8784 Human
- Entrez Gene: 939 Human
- Entrez Gene: 940 Human
- Entrez Gene: 958 Human
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Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling GITR with ab237714 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on the human tonsil tissue is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Formalin-fixed, paraffin-embedded human tonsil tissue stained for TNFSF18 using ab237714 at 0.5 μg/ml in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling TMEM173 with ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human colon cancer (PMID: 26748708) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling TMEM173 with ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in human tonsil (PMID:28874664) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue labeling TMEM173 with ab239074 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in basal cells of human prostate hyperplasia is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining in megakaryocytes of human bone marrow (PMID: 15693793) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling CD226 with ab212076 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining of platelets and endothelium in human spleen is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Perform heat-induced antigen retrieval using ab208572 (Universal HIER antigen retrieval reagent).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human stomach tissue labelling CD27 with ab131254 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD27 with purified ab131254 at 1/1500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections) analysis of human tonsil tissue labelling CD27 with ab131254 at a dilution of 1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue showing negative staining with purified ab131254 at 1/800.
Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD28 with ab243228 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining on subsets of immune cells of human tonsil (PMID: 7489738) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human multiple myeloma tissue labeling CD28 with ab243228 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on human Multiple Myeloma cells. (PMID: 7522634) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD40 with ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in human tonsil tissue (PMID:20593977; PMID:7507299) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labeling CD40 with ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in human diffuse large B-cell lymphoma tissue (PMID: 20593977) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD40 with ab224639 at 1/250 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and cytoplasmic staining in Reed-Sternberg cells and tumor cells of human Hodgkin's lymphoma (PMID:7507299) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human thymus tissue labeling CD134 / OX40L with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on T cells of human thymus (PMID/8547142). Counterstained with Hematoxylin.
Secondary antibody only control/ Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CD134 / OX40L receptor with ab264466 at 1/4000 dilution (0.13 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on human Hodgkin's lymphoma (PMID/8547142). Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in tumor infiltrating lymphoid cells of human cervix cancer (PMID: 23802069) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human stomach (magnification x200) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Stimulatory immune checkpoint panel - human IHC (ab278178)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling ICOS with ab224644 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and membranous staining in a population of lymphoid cells of human tonsil (PMID: 20207847), (magnification x200) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).