Stearoylated Protein Assay Kit (Red) (ab273280)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Stearoylated Protein Assay Kit (Red)
See all Stearoylated Protein kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Stearoylated Protein Assay Kit (Red) (ab273280) offers a powerful method for imaging localization, trafficking, and dynamics of Stearoylated proteins or detection by FACS for quantitative studies. Labeled stearoylated proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis.
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Tested applications
Suitable for: FM, Flow Cytmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative Solution 1 x 10ml Fluorescent Azide (100X) 1 x 100µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Stearic Acid Label (1000X) 1 x 10µl Total DNA Stain (1000X) 1 x 20µl Wash Buffer (10X) 1 x 25ml
Images
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Metabolic labeling of Stearoylated Protein on proliferating Jurkat cells
Metabolic labeling of Stearic Acid on proliferating Jurkat cells. 1 x 106 cells were incubated overnight with fresh aliquots of media containing Stearic Acid Label: Flow cytometry analysis of Negative Control (Black), Background (Fluorescent Azide only, Green), Positive Control (Stearic Acid Label and Fluorescent Azide, Red). Signal measured in FL-2 channel clearly shows the Stearic Acid Labeling of Protein.
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Metabolic labeling of Stearoylated Protein on proliferating HeLa cellsMetabolic labeling of Stearoylated Protein on proliferating HeLa cells. 1 x 105 cells incubated overnight with fresh aliquots of media containing Stearic Acid Label: Upper panel corresponds to the Fluorescent Azide only Background fluorescence on HeLa cells. The lower panel shows that the red fluorescence corresponds to the Stearic Acid Label labeling reaction. Nuclear staining in both panels confirms that green signal is a result of Stearic Acid Label incorporation.
