Stearoylated Protein Assay Kit (Green) (ab273283)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Stearoylated Protein Assay Kit (Green)
See all Stearoylated Protein kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Assay duration
Multiple steps standard assay -
Product overview
Stearoylated Protein Assay Kit (Green) (ab273283) a highly specific, simple and robust method for labeling and detection of Stearoylated proteins. The kit uses Alkynyl Stearic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Stearoylated proteins, or detection by FACS for quantitative studies. Labeled Stearoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis.
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Tested applications
Suitable for: FM, Flow Cytmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative Solution 1 x 10ml Fluorescent Azide (100X) 1 x 100µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Stearic Acid Label (1000X) 1 x 10µl Total DNA Stain (1000X) 1 x 20µl Wash Buffer (10X) 1 x 25ml
Images
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Metabolic labeling of Stearoylated Protein on proliferating HeLa cells.
Example data.
1 x 105 cells incubated overnight with fresh aliquots of media containing Stearoylated Protein Label: Upper panel corresponds to the Fluorescent Azide only Background fluorescence on HeLa cells. The lower panel shows that the red fluorescence corresponds to the Stearoylated Protein Label labeling reaction. Nuclear staining in both panels confirms that green signal is a result of Stearoylated Protein Label incorporation.
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Metabolic labeling of Stearoylated Protein on proliferating Jurkat cellsExample data.
1 x 106 cells were incubated overnight with fresh aliquots of media containing Stearoylated Protein Label: Flow cytometry analysis of Negative Control (Black), Background (Fluorescent Azide only, Green), Positive Control (Stearoylated Protein Label and Fluorescent Azide, Red). Signal measured in FL-1 channel clearly shows the Stearoylated Protein Labeling of Protein.