Soluble Epoxide Hydrolase Assay Kit (ab240999)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate, Purified protein, Tissue Lysate
Overview
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Product name
Soluble Epoxide Hydrolase Assay Kit -
Detection method
Fluorescent -
Sample type
Cell Lysate, Purified protein, Tissue Lysate -
Product overview
Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activity.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Fluorescence Standard 1 vial sEH Assay Buffer 1 x 25ml sEH Inhibitor 5X 1 x 100µl sEH Positive Control 1 vial sEH Substrate 1 x 200µl -
Research areas
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Function
Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water. -
Tissue specificity
Found in liver. -
Involvement in disease
Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. -
Sequence similarities
Belongs to the peptidase S33 family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Alternative names
- EPHX
- EPHX 1
- Ephx1
see all
Images
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Fluorescence standard curve for sEH metabolite
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sEH activity in rat liver tissue lysate in absence and presence of inhibitor. -
Enzyme kinetics in presence and absence of inhibitor for sEH positive control, and rat liver lysate (3.6 µg protein per well).
