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Soluble Epoxide Hydrolase Assay Kit (ab240999)

Key features and details

  • Detection method: Fluorescent
  • Platform: Microplate reader
  • Sample type: Cell Lysate, Purified protein, Tissue Lysate

Overview

  • Product name

    Soluble Epoxide Hydrolase Assay Kit

  • Detection method

    Fluorescent

  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate

  • Product overview

    Soluble Epoxide Hydrolase Assay Kit (ab240999) is a microplate based fluorometric kit for measuring Soluble Epoxide Hydrolase (sEH) activity in cells and tissues as well as purified protein. It is based on the ability of sEH to hydrolyze a non-fluorescent substrate to a fluorescent product. The kit includes a specific inhibitor for soluble epoxide hydrolase, since the substrate can be hydrolyzed by non-specific hydrolases that are present in cell and tissue lysates. Specific sEH activity can be obtained by subtracting the activity in presence of sEH inhibitor form the total activity.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Fluorescence Standard 1 vial
    sEH Assay Buffer 1 x 25ml
    sEH Inhibitor 5X 1 x 100µl
    sEH Positive Control 1 vial
    sEH Substrate 1 x 200µl

  • Research areas

  • Function

    Biotransformation enzyme that catalyzes the hydrolysis of arene and aliphatic epoxides to less reactive and more water soluble dihydrodiols by the trans addition of water.

  • Tissue specificity

    Found in liver.

  • Involvement in disease

    Note=In some populations, the high activity haplotype tyr113/his139 is overrepresented among women suffering from pregnancy-induced hypertension (pre-eclampsia) when compared with healthy controls.
    Defects in EPHX1 are a cause of familial hypercholanemia (FHCA) [MIM:607748]. FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption.

  • Sequence similarities

    Belongs to the peptidase S33 family.

  • Cellular localization

    Microsome membrane. Endoplasmic reticulum membrane.
  • Information by UniProt

  • Alternative names

    • EPHX
    • EPHX 1
    • Ephx1
    • EPOX
    • Epoxide hydratase
    • Epoxide hydrolase 1
    • Epoxide hydrolase 1 microsomal (xenobiotic)
    • Epoxide hydroxylase 1
    • Epoxide hydroxylase 1 microsomal (xenobiotic)
    • HYEP_HUMAN
    • HYL1
    • MEH
    • Microsomal epoxide hydrolase
    see all

Images

  • Fluorescence standard curve for sEH metabolite
    Fluorescence standard curve for sEH metabolite
  • sEH activity in rat liver tissue lysate in absence and presence of inhibitor.
    sEH activity in rat liver tissue lysate in absence and presence of inhibitor.
  • Enzyme kinetics in presence and absence of inhibitor for sEH positive control, and rat liver lysate (3.6 µg protein per well).
    Enzyme kinetics in presence and absence of inhibitor for sEH positive control, and rat liver lysate (3.6 µg protein per well).

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