Sodium Assay Kit (Colorimetric) (ab211096)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Saliva, Serum, Urine
- Sensitivity: 25 µM
Overview
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Product name
Sodium Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum -
Assay type
Quantitative -
Sensitivity
25 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Sodium Assay Kit (Colorimetric) (ab211096) provides a convenient two-step method to detect sodium ions (Na+) present in serum, urine and saliva. In this assay, sodium ions present in the sample are used by the enzyme β-galactosidase to produce an intermediate product, which reacts with a developer to generate a color signal that can be detect at OD = 405 nm.
This kit can detect as low as 25 µM sodium in biological fluids. Endogenous mono-, di-, and trivalent ions, ascorbic acid, creatinine, glucose, urea, and bilirubin do not interfere with the assay.
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Notes
Sodium (Na+) is one of the most important electrolytes along with chloride, calcium and potassium. Sodium plays vital roles in the maintenance normal cell functions such as plasma volume, pH balance or transmission of nerve impulses. Healthy individuals can absorb sodium ingested in food, and kidneys maintain proper sodium balance by excreting its excess in urine. Normal Sodium intake has been defined to be between 200-500 mg/day. Hyponatremia (low sodium concentration in blood) can occur in patients with nephrotic syndrome, excessive vomiting and diarrhea, while Hypernatremia (high sodium concentration in blood) is developed in patients suffering from liver diseases, burns, and pregnancy. Traditionally, sodium concentration in clinical settings is determined by potentiometric, gravimetry, photometry, titrimetry and flame atomic emission spectroscopy, but these methods require expensive and complex protocols that need to be performed by trained personnel.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Na Assay Buffer 1 x 25ml Substrate 1 x 5ml β-Gal 1 x 15µl DTT (1 M) 1 x 0.25ml Na Developer 1 x 10ml Na Standard (1.5 M) 1 x 1ml
Images
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Sodium Assay Kit (Colorimetric) (ab211096)
Typical Sodium standard calibration curve.
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Sodium Assay Kit (Colorimetric) (ab211096)Assay Specificity: Sodium, and other mono, di and trivalent cations (15 nmol/0.15 mM each) were tested to evaluate possible interferences. Interferences were found to be
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Sodium Assay Kit (Colorimetric) (ab211096)Estimation of sodium in human pooled serum off-the-clot (5 µL; 1:50 dilution) and human urine (10 µL; 1:100 dilution).