SNAP25 ELISA Kit (ab256394)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 19 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
SNAP25 ELISA Kit
See all SNAP25 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 5.7% Inter-assay Sample n Mean SD CV% Extract 3 4.3% -
Sample type
Tissue Extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
19 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 102 94% - 106% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
SNAP25 ELISA Kit (ab256394) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of SNAP25 protein in tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate SNAP25 with 19 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
SNAP25, Synaptosomal nerve-associated protein 25, is a t-snare protein involved in docking and exocytosis of vesicles and neurotransmitter release. It docks with its coreceptor on the vesicle, v-snare, and syntaxin to form a quaternary alpha helix that stabilizes the fusion awaiting a calcium-regulated neurotransmitter exocytosis event. Decreased SNAP25 levels are associated with schizophrenia and attention deficit hyperactivity disorder. Human, mouse and rat SNAP25 protein have 100% conserved amino acid sequence.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human SNAP25 Capture Antibody 1 x 600µl 10X Human SNAP25 Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human SNAP25 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. -
Tissue specificity
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. -
Sequence similarities
Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains. -
Post-translational
modificationsPalmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association. -
Cellular localization
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region. - Information by UniProt
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Alternative names
- bA416N4.2
- Bdr
- CMS18
see all -
Database links
- Entrez Gene: 6616 Human
- Omim: 600322 Human
- SwissProt: P60880 Human
- Unigene: 167317 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human, mouse, and rat SNAP25 standard curve in 1X Cell Extraction Buffer PTR.The SNAP25 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native SNAP25 in human cerebellum tissue extract based on a 200 µg/mL extract load.The concentrations of SNAP25 were measured in duplicate and interpolated from the SNAP25 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean SNAP25 concentration was determined to be 1591.46 pg/mL.
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Interpolated concentrations of native SNAP25 in human cerebellum, mouse brain, and rat brain tissue extracts based on a 50 µg/mL extract load.The concentrations of SNAP25 were measured in duplicate and interpolated from the SNAP25 standard curve.
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Sandwich ELISA - SNAP25 ELISA Kit (ab256394)To learn more about the advantages of recombinant antibodies see here.
