Sialic Acid (NANA) Assay Kit (ab83375)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture media, Cell culture supernatant, Urine
- Sensitivity: 1 µM
Overview
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Product name
Sialic Acid (NANA) Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Cell culture media -
Assay type
Quantitative -
Sensitivity
1 µM -
Range
0.1 µM - 10 µM -
Assay time
0h 40m -
Product overview
Sialic Acid (NANA) Assay Kit (ab83375) is a simple and convenient kit for measuring free sialic acid (mainly N-acetylneuraminic acid (NANA)) in biological samples.
The sialic acid assay uses an enzyme coupled reaction in which oxidation of free sialic acid creates an intermediate that reacts stoichiometrically with the probe to generate a product that can detected by absorbance (OD = 570 nm) or fluorescence (Ex/Em=535/587 nm).
The kit measures sialic acid in the linear range of 0.1 to 10 nmol with a detection sensitivity ~1 µM concentration.
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Notes
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been shown recently that sialic acid level may be associated with developmental and pathological stages.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Sialic Acid Assay Buffer WM 1 x 25ml Sialic Acid Converting Enzyme (lyophilized) Purple 1 vial Sialic Acid Development Mix (lyophilized) Green 1 vial Sialic Acid Probe (in DMSO) Red 1 x 200µl Sialic Acid Standard (10 umol) (lyophilized) Yellow 1 vial -
Research areas
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Relevance
Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone. It is also the name for the most common member of this group, N-acetylneuraminic acid (NANA). Sialic acids are found widely distributed in animal tissues and to a lesser extent in other species ranging from plants and fungi to yeasts and bacteria, mostly in glycoproteins and gangliosides. It has been show recently that sialic acid level may associate with developmental and pathological stages. -
Alternative names
- N acetylneuraminic acid
- NANA
Images
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Functional Studies - Sialic Acid (NANA) Assay Kit (ab83375) Mudd, Austin T et al., Frontiers in nutrition?vol. 3 39., Fig 3, doi:10.3389/fnut.2016.00039Concentrations of free SA decreased (P
Functional studies - Sialic Acid 5NANA) Assay Kit (ab83375)Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated
Functional Studies - Sialic Acid (NANA) Assay Kit (ab83375)Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375. -
Functional studies - Sialic Acid 5NANA) Assay Kit (ab83375)
Standard curve (fluorimetric) : mean of duplicates (+/-SD) with background readings subtrated
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Functional Studies - Sialic Acid (NANA) Assay Kit (ab83375)Examples of colorimetric and fluorometric standard Sialic acid curves using ab83375.
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