Recombinant rat PDE1B protein (ab198476)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 35% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant rat PDE1B protein
See all PDE1B proteins and peptides -
Biological activity
Specific Activity: ≥ 6800 pmol/min/µg
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198476 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
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Purity
>= 35 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MELSPRSPPEMLESDCPSPLELKSAPSKKMWIKLRSLLRYMVKQLENGEV NIEELKKNLEYTASLLEAVYIDETRQILDTEDELRELRSDAVPSEVRDWL ASTFTQQTRAKGRRAEEKPKFRSIVHAVQAGIFVERMFRRTYTAVGPTYS TAVHNCLKNLDVWCFDVFSLNRAADDHALRTIVFELLTRHSLISRFKIPT VFLMSFLEALETGYGKYKNPYHNQIHAADVTQTVHCFLLRTGMVHCLSEI EVLAIIFAAAIHDYEHTGTTNSFHIQTKSECAILYNDRSVLENHHISSVF RMMQDDEMNIFINLTKDEFVELRALVIEMVLATDMSCHFQQVKTMKTALQ QLERIDKSKALSLLLHAADISHPTKQWSVHSRWTKALMEEFFRQGDKEAE LGLPFSPLCDRTSTLVAQSQIGFIDFIVEPTFSVLTDVAEKSVQPLTDDD SKSKSQPSFQWRQPSLDVDVGDPNPDVVSFRSTWTKYIQENKQKWKERAA SGITNQMSIDELSPCEEEAPSSPAEDEHNQNGNLD -
Predicted molecular weight
89 kDa including tags -
Amino acids
1 to 535 -
Tags
His tag N-Terminus , GST tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 1.36% Imidazole
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant rat PDE1B protein (ab198476)
Example of specific activity of ab198476
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SDS-PAGE - Recombinant rat PDE1B protein (ab198476)10% SDS-PAGE analysis of ab198476 with Coomassie staining.
Lane 1: 2.7 μg ab198476
Lane 2: Protein marker
