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Cell Biology Proteolysis / Ubiquitin Proteolytic enzymes Serine protease DPPs

Anti-DPP3 antibody (ab217127)

Anti-DPP3 antibody (ab217127)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DPP3
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DPP3 antibody
    See all DPP3 primary antibodies
  • Description

    Rabbit polyclonal to DPP3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human DPP3 aa 650 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9NY33

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HeLa, 293T, Jurkat, TCMK1 and NIH 3T3 cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7.0 to 8.0
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab217127 was affinity purified using an epitope specific to DPP3 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Serine protease
    • DPPs

Images

  • Western blot - Anti-DPP3 antibody (ab217127)
    Western blot - Anti-DPP3 antibody (ab217127)
    All lanes : Anti-DPP3 antibody (ab217127) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 83 kDa


    Exposure time: 30 seconds


    Lysis buffer: NETN

  • Immunoprecipitation - Anti-DPP3 antibody (ab217127)
    Immunoprecipitation - Anti-DPP3 antibody (ab217127)

    Detection of DPP3 in Immunoprecipitates of 293T whole cell lysates (1 mg for IP, 20% of IP loaded) using ab217127 at 6 µg/mg lysate for IP (Lane 1). For WB detection ab217127 was used at 1µg/ml. Lane 2 Control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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