Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant rat Oncostatin M/OSM protein (Active) (ab200252)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 96% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, HPLC, SDS-PAGE

Description

  • Product name

    Recombinant rat Oncostatin M/OSM protein (Active)
    See all Oncostatin M/OSM proteins and peptides

  • Biological activity

    Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.

  • Purity

    > 96 % SDS-PAGE.
    Purity > 96 % by SDS-PAGE and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q65Z15

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Sequence

      KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR

    • Predicted molecular weight

      24 kDa

    • Amino acids

      26 to 239

    • Additional sequence information

      This product is for the mature full length protein. The signal peptide is not included.

    • Specifications

    Our Abpromise guarantee covers the use of ab200252 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Functional Studies

      HPLC

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Reconstitute for long term storage.

      Constituents: 99% PBS, 0.1% Tween

      Tween-80.

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    • Reconstitution
      We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at = -20 °C. Further dilutions should be made in appropriate buffered solutions.

    General Info

    • Alternative names

      • MGC20461
      • ONCM_HUMAN
      • Oncostatin M
      • Oncostatin-M
      • OSM
      see all

    • Function

      Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST).

    • Sequence similarities

      Belongs to the LIF/OSM family.

    • Cellular localization

      Secreted.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab200252? Please let us know so that we can cite the reference in this datasheet.

ab200252 has not yet been referenced specifically in any publications.

Preparation and Storage

  • MGC20461
  • ONCM_HUMAN
  • Oncostatin M

Images

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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