Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant rat FGF1 protein (ab126927)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, HPLC

Description

  • Product name

    Recombinant rat FGF1 protein
    See all FGF1 proteins and peptides

  • Biological activity

    Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED50 is = 0.1 ng/ml, in the presence of 10 µg/ml heparin, corresponding to a specific activity of = 1 x 107 units/mg.

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% by SDS-PAGE gel and HPLC analyses.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    P61149

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Rat

    • Sequence

      MFNLPLGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SAGEVYIKGT ETGQYLAMDT EGLLYGSQTP NEECLFLERL EENHYNTYTS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

    • Predicted molecular weight

      16 kDa

    • Amino acids

      16 to 155

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab126927 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        Functional Studies

        SDS-PAGE

        HPLC

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C.

        Constituents: 0.16% Sodium phosphate, 0.88% Sodium chloride, 0.2% CHAPS

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Centrifuge the vial prior to opening. Reconstitute in 5mM Sodium Phosphate, pH 7.2 to a concentration of =0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20oC to -80oC.

      General Info

      • Alternative names

        • Acidic fibroblast growth factor
        • aFGF
        • Beta endothelial cell growth factor
        • Beta-endothelial cell growth factor
        • ECGF
        • ECGF beta
        • ECGF-beta
        • ECGFA
        • ECGFB
        • Endothelial Cell Growth Factor alpha
        • Endothelial Cell Growth Factor beta
        • FGF 1
        • FGF alpha
        • Fgf1
        • FGF1_HUMAN
        • FGFA
        • Fibroblast growth factor 1
        • Fibroblast Growth Factor 1 Acidic
        • GLIO703
        • HBGF 1
        • HBGF-1
        • HBGF1
        • Heparin binding growth factor 1
        • Heparin binding growth factor 1 precursor
        • Heparin-binding growth factor 1
        see all

      • Function

        The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.

      • Sequence similarities

        Belongs to the heparin-binding growth factors family.

      • Cellular localization

        Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
      • Information by UniProt

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab126927? Please let us know so that we can cite the reference in this datasheet.

    ab126927 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Alternative names

      • Acidic fibroblast growth factor
      • aFGF
      • Beta endothelial cell growth factor
      • Beta-endothelial cell growth factor
      • ECGF
      • ECGF beta
      • ECGF-beta
      • ECGFA
      • ECGFB
      • Endothelial Cell Growth Factor alpha
      • Endothelial Cell Growth Factor beta
      • FGF 1
      • FGF alpha
      • Fgf1
      • FGF1_HUMAN
      • FGFA
      • Fibroblast growth factor 1
      • Fibroblast Growth Factor 1 Acidic
      • GLIO703
      • HBGF 1
      • HBGF-1
      • HBGF1
      • Heparin binding growth factor 1
      • Heparin binding growth factor 1 precursor
      • Heparin-binding growth factor 1
      see all

    • Function

      The heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.

    • Sequence similarities

      Belongs to the heparin-binding growth factors family.

    • Cellular localization

      Secreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1.
    • Information by UniProt

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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