Recombinant rat CERK protein (Active) (ab271469)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: = 65% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant rat CERK protein (Active)
See all CERK proteins and peptides -
Biological activity
Specific activity ≥20 pmole/min/μg.
CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide.
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Purity
= 65 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Sequence
MGAMGAAEPL QSVLWVKQQR CAVSLEPARA LLRWWRSPEP GPSAPDPDAY SVPVSEIIAV EEKEVHQKQS SNGRWHRMEN PFAFTVHCVK RARHHRWKWA RVTFWSADEQ LCQLWLQTLR ELLENLTSRP KHLLVFINPF GGKGQGKHIY EKKVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF SEVLHGVIGK TQQSAGVDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG TNDAETSALH IIIGDSLAID VSSVHHNSTL LRYSVSLLGY GFYGDLIKDS EKKRWMGLVR YDFSGLKAFL SHQYYEGTVS FLPAQHTVGS PRDQKPCRAG CSVCRQSKRQ LEEEQKKALY GLENAEEVEE WQVVCGKFLA INSTNMSCAC PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFGFTFV EVYRVKKFQF TSKHVEDDDN DLKELEKQKF GQICKDNPPC ACPTSRSSWN CDGEVLHSPA IEVRVHCQLV RLFARGIEEE S -
Predicted molecular weight
61 kDa including tags -
Amino acids
1 to 531 -
Tags
DDDDK tag C-Terminus
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Preparation and Storage
- Acylsphingosine kinase
- Ceramide kinase
- Cerk
Images
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Functional Studies - Recombinant Rat CERK protein (Active) (ab271469)
Functional analysis of ab271469.
Specific activity ≥20 pmole/min/μg.
CERK activity was measured at 37oC for 25 min in a buffer (20 mM HEPES pH7.2, 80 mM KCl, 3 mM CaCl2, 10 mM MgCl2, 2 mM DTT, 5 μM ATP) using a μ-Octylglucoside mixed micellar assay with 100 μM Ceramide.
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SDS-PAGE - Recombinant Rat CERK protein (Tagged) (ab271469)SDS-PAGE analysis of ab271469.