Anti-Tubulin antibody - Loading Control (ab56676)
Key features and details
- Mouse monoclonal to Tubulin - Loading Control
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Tubulin antibody - Loading Control
See all Tubulin primary antibodies -
Description
Mouse monoclonal to Tubulin - Loading Control -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanWB Human
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Immunogen
Recombinant full length protein corresponding to Human Tubulin aa 1-451.
Database link: P68363 -
General notes
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading... -
Purity
Tissue culture supernatant -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Tubulin antibody - Loading Control (ab56676)
Tubulin antibody (ab56676) at 1ug/lane + A-431 cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tubulin antibody - Loading Control (ab56676)Tubulin antibody (ab56676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human prostate.
This image was generated using the ascites version of the product.
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Western blot - Anti-Tubulin antibody - Loading Control (ab56676) This image is courtesy of an Abreview submitted by Xun AiAll lanes : Anti-Tubulin antibody - Loading Control (ab56676) at 1 µg/ml (in TPBS for 18 hours at 4°C)
Lane 1 : Whole tissue lysate of rabbit heart at 20 µg
Lane 2 : Whole tissue lysate of rabbit heart at 40 µg
Lane 3 : Whole tissue lysate of rabbit heart at 60 µg
Lane 4 : Whole tissue lysate of rabbit heart at 80 µg
Lane 5 : Whole tissue lysate of rabbit heart at 100 µg
Lane 6 : Whole tissue lysate of rabbit heart at 120 µg
Secondary
All lanes : An HRP-conjugated Donkey anti-mouse IgG polyclonal at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 47 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking Step : 5% BSA for 2 hours at 23°C
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-Tubulin antibody - Loading Control (ab56676)Overlay histogram showing HeLa cells stained with ab56676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56676, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
