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Astana Biomed Group, an authorized Abcam distributor in Central Asia

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules

Anti-EML4 antibody (ab86062)

Anti-EML4 antibody (ab86062)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to EML4
  • Suitable for: WB, IP, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-EML4 antibody
    See all EML4 primary antibodies
  • Description

    Rabbit polyclonal to EML4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Guinea pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 150 and 200 of human EML4 (NP_061936.2)

  • Positive control

    • HeLa whole cell lysate.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.8
    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab86062 was affinity purified using an epitope specific to EML4 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Cytoskeleton
    • Microtubules

Images

  • Western blot - Anti-EML4 antibody (ab86062)
    Western blot - Anti-EML4 antibody (ab86062)
    All lanes : Anti-EML4 antibody (ab86062) at 1/0.04 dilution

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Predicted band size: 109 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab86062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EML4 antibody (ab86062)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lin plastica stomach cancer tissue labelling EML4 with ab86062 at 1/200 (1µg/ml). Detection: DAB.
  • Immunoprecipitation - Anti-EML4 antibody (ab86062)
    Immunoprecipitation - Anti-EML4 antibody (ab86062)
    Detection of EML4 by Western Blot of Immunprecipitate.
    ab86062 at 0.4µg/ml staining EML4 in HeLa whole cell lysate immunoprecipitated using ab86062 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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