Recombinant Mouse IgG1 protein (ab180055)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Suitable for: SDS-PAGE
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Product name
Recombinant Mouse IgG1 protein
See all IgG1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Endotoxin level
Expression system
HEK 293 cellsAccession
Protein length
Protein fragmentAnimal free
NoNature
Recombinant-
Species
Mouse -
Sequence
VPEVSSVFIFPPKPKDVLTITLTPKVTCVVVDISKDDPEVQFSWFVDDVE VHTAQTQPREEQFNSTFRSVSELPIMHQDWLNGKEFKCRVNSAAFPAPIE KTISKTKGRPKAPQVYTIPPPKEQMAKDKVSLTCMITDFFPEDITVEWQW NGQPAENYKNTQPIMDTDGSYFVYSKLNVQKSNWEAGNTFTCSVLHEGLH NHHTEKSLSHSPGK -
Predicted molecular weight
24 kDa -
Amino acids
111 to 324 -
Additional sequence information
The fragment corresponds to the CH2 and CH3 domains. Genbank entry: AAK53870.1.
Associated products
Specifications
Our Abpromise guarantee covers the use of ab180055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.4
Constituents: 5% Trehalose, 0.61% Tris, 0.75% Glycine, L-Arginine, Sodium chloride
Lyophilized from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
General Info
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Alternative names
- Ig gamma 1 chain C region
- IgG heavy chain locus
- IGHG1
see all -
Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Cellular localization
Secreted. Cell membrane; Single-pass membrane protein
Images
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SDS-PAGE - Recombinant Mouse IgG1 protein (Fc Chimera) (ab180055)
SDS-PAGE analysis of ab180055 under reducing conditions. Gel stained overnight with Coomassie Blue. The protein has a calculated MW of 24.3 kDa. The reduced protein migrates as 28-30 kDa due to glycosylation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab180055 has not yet been referenced specifically in any publications.
Preparation and Storage
-
Alternative names
- Ig gamma 1 chain C region
- IgG heavy chain locus
- IGHG1
see all -
Relevance
There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). -
Cellular localization
Secreted. Cell membrane; Single-pass membrane protein
Images
-
SDS-PAGE - Recombinant Mouse IgG1 protein (Fc Chimera) (ab180055)
SDS-PAGE analysis of ab180055 under reducing conditions. Gel stained overnight with Coomassie Blue. The protein has a calculated MW of 24.3 kDa. The reduced protein migrates as 28-30 kDa due to glycosylation.
