Native Human IgG1 protein (ab90283)
Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: SDS-PAGE
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Product name
Native Human IgG1 protein
See all IgG1 proteins and peptides -
Purity
> 95 % SDS-PAGE. -
Expression system
Native -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Native -
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Species
Human -
Predicted molecular weight
146 kDa -
Additional sequence information
This product is the whole IgG molecule composed of 2 heavy chains and 2 light chains, joined via disulfide bonds.
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.87% Sodium chloride, 0.3% Sodium phosphate monobasic, monohydrate
Images
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ELISA - Native Human IgG1 protein (ab90283)
The wells were coated with ab200699 at 5µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG1 (ab90283) was then added starting at 100 µg/ml and plasma/serum at 1:50 and gradually diluted 1:4, 50µl/well for 2h. Ab201485 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
