Recombinant mouse GM-CSF protein (Active) (ab259385)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, HPLC, MS, SDS-PAGE, Cell Culture
Preparation and Storage
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Alternative names
- Colony stimulating factor
- Colony Stimulating Factor 2
- Colony stimulating factor 2 (granulocyte-macrophage)
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Function
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. -
Sequence similarities
Belongs to the GM-CSF family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant mouse GM-CSF protein (Active) (ab259385)
Fully biologically active when compared to standard. Determined by the dose dependant proliferation of MC/9 cells.
ED50 is ≤0.04 ng/mL, corresponding to a specific activity of 2.50 x 107 units/mg.
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SDS-PAGE - Recombinant mouse GM-CSF protein (Active) (ab259385)SDS-PAGE analysis of ab259385.
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HPLC - Recombinant mouse GM-CSF protein (Active) (ab259385)Purity >= 95%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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Mass Spectrometry - Recombinant mouse GM-CSF protein (Active) (ab259385)Mass – 1 Da (+237.23 Da: Pi[3]; + 203 Da: HexNAc[1]; +162 Da: Hex[1]) (Calc. mass 14211.77 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).