Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TRAF2 antibody [EPR7064] (ab167163)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7064] to TRAF2
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Product name

Anti-TRAF2 antibody [EPR7064]
See all TRAF2 primary antibodies
Description

Rabbit monoclonal [EPR7064] to TRAF2
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment corresponding to Human TRAF2.
Positive control
- Purchase matching WB positive control:Human TRAF2 full length protein
- WB: HEK-293T. Molt-4, 293T, Raji and HeLa whole cell lysate (ab150035). IHC-P: Human kidney tissue ICC: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7064
Isotype

IgG
Research areas

Western blot - Anti-TRAF2 antibody [EPR7064] (ab167163)

All lanes : Anti-TRAF2 antibody [EPR7064] (ab167163) at 1/1000 dilution

Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TRAF2 knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 55 kDa
Observed band size: 55 kDa

Lanes 1- 2: Merged signal (red and green). Green - ab167163 observed at 55 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

ab167163 was shown to react with TRAF2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266060 (knockout cell lysate ab257759) was used. Wild-type HEK-293T and TRAF2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab167163 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRAF2 antibody [EPR7064] (ab167163)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling TRAF2 with ab167163 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-TRAF2 antibody [EPR7064] (ab167163)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: TRAF2 knockout HAP1 cell lysate (20 µg)
Lane 3: Human skeletal muscle tissue lysate (20 µg)
Lane 4: U20S cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab167163 observed at 58 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab167163 was shown to recognize TRAF2 when TRAF2 knockout samples were used, along with additional cross-reactive bands. Wild-type and TRAF2 knockout samples were subjected to SDS-PAGE. ab167163 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-TRAF2 antibody [EPR7064] (ab167163)

All lanes : Anti-TRAF2 antibody [EPR7064] (ab167163) at 1/1000 dilution

Lane 1 : Molt-4 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TRAF2 antibody [EPR7064] (ab167163)

Immunofluorescent analysis of HeLa cells labeling TRAF2 with ab167163 at 1/100 dilution.
Anti-TRAF2 antibody [EPR7064] (ab167163)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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