Human MASPIN Matched Antibody Pair Kit (ab219539)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 62.5 pg/ml - 4000 pg/ml
Overview
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Product name
Human MASPIN Matched Antibody Pair Kit
See all MASPIN kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
11.1 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human MASPIN Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human MASPIN.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab218269. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human MASPIN Capture Antibody 2 x 50µg 1 x 50µg Human MASPIN Detector Antibody 2 x 12.5µg 1 x 12.5µg Human MASPIN Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Normal mammary epithelial cells. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Maspin
- Ovalbumin
- Peptidase inhibitor 5
see all -
Database links
- Entrez Gene: 5268 Human
- Omim: 154790 Human
- SwissProt: P36952 Human
- Unigene: 55279 Human
Images
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Human MASPIN Standard Curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human MASPIN Matched Antibody Pair Kit (ab219539)To learn more about the advantages of recombinant antibodies see here.
