Human MASPIN ELISA Kit (ab218269)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8 pg/ml
- Range: 0.11 ng/ml - 7 ng/ml
- Sample type: Cell culture extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human MASPIN ELISA Kit
See all MASPIN kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 8 3.8% Inter-assay Sample n Mean SD CV% Overall 3 1.4% -
Sample type
Cell culture extracts -
Assay type
Sandwich (quantitative) -
Sensitivity
8 pg/ml -
Range
0.11 ng/ml - 7 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 108 107% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human -
Product overview
Human MASPIN ELISA Kit (ab218269) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MASPIN protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human MASPIN with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
MASPIN (Mammary Serine Protease Inhibitor) is a 375 amino acid glycosylated protein encoded by the gene SERPINB5. It belongs to a family of proteins known as serpins (serine protease inhibitor), which function as regulators of protein breakdown by acting as suicide inhibitors of the catalytic activity of proteinases. Inhibitory serpins undergo a transition between a stressed and relaxed confirmation, where the stressed confirmation binds to the catalytic serine residue of the protease. MASPIN is thought to interact with extracellular matrix components, glutathione S-transferase, and interferon regulatory factor 6. It was originally thought to play a role in tumor metastasis, but the exact function is unknown.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human MASPIN Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 4BI 1 x 6ml Human MASPIN Capture Antibody (Lyophilized) 1 vial Human MASPIN Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. -
Tissue specificity
Normal mammary epithelial cells. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Cellular localization
Secreted > extracellular space. - Information by UniProt
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Alternative names
- Maspin
- Ovalbumin
- Peptidase inhibitor 5
see all -
Database links
- Entrez Gene: 5268 Human
- Omim: 154790 Human
- SwissProt: P36952 Human
- Unigene: 55279 Human
Images
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Other - Human MASPIN ELISA Kit (ab218269)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human MASPIN standard curve in 1X Cell Extraction Buffer PTRBackground-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native MASPIN in human PC-3 cell extract based on a 1,000 µg/mL extract load.The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve, and corrected for sample dilution. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 57.5 ng/mL in PC-3 cell extract.
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Interpolated concentrations of native MASPIN in human PC-3, A431, HL-60, and Hela cell extract based on a 10 µg/mL extract load.The concentrations of MASPIN were measured in duplicate, interpolated from the MASPIN standard curve. The interpolated, dilution factor-corrected values are plotted (mean +/- SD, n=2). The mean MASPIN concentration was determined to be 55 ng/mL, 93 ng/mL, and non-detectable in PC-3, A431, HL-60, and HeLa cell extract, respectively.
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Sandwich ELISA - Human MASPIN ELISA Kit (ab218269)To learn more about the advantages of recombinant antibodies see here.
