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Human HTR3D (5HT3D Receptor) knockout A549 cell lysate (ab263231)

Price and availability

318 288 ₸

Availability

Order now and get it on Friday March 05, 2021

Human HTR3D (5HT3D Receptor) knockout A549 cell lysate (ab263231)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

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Overview

  • Product name

    Human HTR3D (5HT3D Receptor) knockout A549 cell lysate
    See all 5HT3D Receptor kits
  • Product overview


    Knockout cell lysate achieved by CRISPR/Cas9.

  • Parental Cell Line

    A549
  • Organism

    Human
  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6 and 2 bp insertion in exon6.
  • Passage number

  • Knockout validation

    Sanger Sequencing
  • Reconstitution notes

    To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

    *Usage of SDS sample buffer is not recommended with these lyophilized lysates.

  • Notes

    Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version - found here. Please refer to our lysis protocol for further details on how our lysates are prepared.

    User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

    Access thousands of knockout cell lysates, generated from commonly used cancer cell lines.
    See here for more information on knockout cell lysates.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262736 - Human HTR3D knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Serotonin Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • More Channels
    • Metabolism
    • Types of disease
    • Obesity
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Target

  • Relevance

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Cellular localization

    Cell membrane; Multi-pass membrane protein. Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
  • Alternative names

    • 5 hydroxytryptamine receptor 3D
    • 5-hydroxytryptamine receptor 3 subunit D
    • 5HT3D
    • HTR3D
    • MGC119636
    • MGC119637
    • Serotonin 5-HT-3D receptor
    • Serotonin receptor 3D
    see all

Properties

  • Storage instructions

    Store at -80°C. Please refer to protocols.
  • Components 1 kit
    ab262736 - Human HTR3D knockout A549 cell lysate 1 x 100µg
    ab255554 - Human wild-type A549 cell lysate 1 x 100µg
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Serotonin Receptors
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • More Channels
    • Metabolism
    • Types of disease
    • Obesity
  • Cell type

    epithelial
  • Disease

    Carcinoma
  • STR Analysis

    Amelogenin X,Y D5S818: 11 D13S317: 11 D7S820: 8, 11 D16S539: 11, 12 vWA: 14 TH01: 8,9.3 TPOX: 8,11 CSF1PO: 10, 12

Images

  • Sanger Sequencing - Human HTR3D knockout A549 cell lysate (ab263231)
    Sanger Sequencing - Human HTR3D knockout A549 cell lysate (ab263231)

    Allele-1: 1 bp deletion in exon6

     

  • Sanger Sequencing - Human HTR3D knockout A549 cell lysate (ab263231)
    Sanger Sequencing - Human HTR3D knockout A549 cell lysate (ab263231)

    Allele-2: 2 bp insertion in exon6

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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