Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: Avi tag C-Terminus, Fc tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse CD80 protein (Active) (Biotin)
See all CD80 proteins and peptides -
Biological activity
Mouse CD80 protein activity was performed in an inhibitor screening assay using mouse CTLA4 coated on a 96-well plate at 100 ng/well, 4°C, overnight. Various amounts of ab271374 (0-50 ng) was incubated for 2 hours at room temperature, followed by streptavidin-HRP for 1 hour at room temperature. Chemiluminescence produced by HRP substrate was measured using a chemiluminescence reader.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
VDEQLSKSVK DKVLLPCRYN SPHEDESEDR IYWQKHDKVV LSVIAGKLKV WPEYKNRTLY DNTTYSLIIL GLVLSDRGTY SCVVQKKERG TYEVKHLALV KLSIKADFST PNITESGNPS ADTKRITCFA SGGFPKPRFS WLENGRELPG INTTISQDPE SELYTISSQL DFNTTRNHTI KCLIKYGDAH VSEDFTWEKP PEDPPDSKN -
Predicted molecular weight
52 kDa including tags -
Amino acids
38 to 246 -
Tags
Avi tag C-Terminus , Fc tag C-Terminus -
Additional sequence information
Extracellular domain fused at the C-terminus of the Fc portion of human IgG1.
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Conjugation
Biotin
Preparation and Storage
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Alternative names
- Activation B7-1 antigen
- B lymphocyte activation antigen B7
- B7
see all -
Function
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28 or CTLA-4 to this receptor. -
Tissue specificity
Expressed on activated B-cells, macrophages and dendritic cells. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)
Mouse CD80 protein activity was performed in an inhibitor screening assay using mouse CTLA4 coated on a 96-well plate at 100 ng/well, 4°C, overnight. Various amounts of ab271374 (0-50 ng) was incubated for 2 hours at room temperature, followed by streptavidin-HRP for 1 hour at room temperature. Chemiluminescence produced by HRP substrate was measured using a chemiluminescence reader.
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SDS-PAGE - Recombinant mouse CD80 protein (Active) (Biotin) (ab271374)SDS-PAGE analysis of 4 µg ab271374.