Recombinant mouse CD73 protein (Active) (ab271446)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, Avi tag C-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant mouse CD73 protein (Active)
See all CD73 proteins and peptides -
Biological activity
Specific Activity: ≥ 20 nmol/min/μg
Assay Description: Assay was performed in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EVPAGKYPFI VTADDGRQVP VVQAYAFGKY LGYLKVEFDD KGNVITSYGN PILLNSSIPE DATIKADINQ WRIKLDNYST QELGRTIVYL DGSTQTCRFR ECNMGNLICD AMINNNLRHP DEMFWNHVSM CIVNGGGIRS PIDEKNNGTI TWENLAAVLP FGGTFDLVQL KGSTLKKAFE HSVHRYGQST GEFLQVGGIH V -
Predicted molecular weight
61 kDa including tags -
Molecular weight information
This protein runs at a higher molecular weight by SDS-PAGE due to glycosylation. -
Amino acids
29 to 549 -
Tags
His tag C-Terminus , Avi tag C-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 0.13% Sodium phosphate, 0.64% Sodium chloride, 0.02% Potassium chloride, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant mouse CD73 protein (Active) (ab271446)
Specific Activity: ≥ 20 nmol/min/μg
Assay Description: Assay was performed in 50 μL assay buffer containing 25 mM Tris, pH 7.4, 5 mM MgCl2 and 100 μM AMP for 15 min at 37°C. Phosphate standard solution was run side-by-side for product quantification.
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SDS-PAGE - Recombinant mouse CD73 protein (Active) (ab271446)SDS-PAGE analysis of 3 µg ab271446.
This protein runs at a higher molecular weight due to glycosylation.