Recombinant mouse CD137 protein (Fc Chimera) (ab220549)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 90% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: Fc tag C-Terminus
- Suitable for: SDS-PAGE, ELISA, Functional Studies
Preparation and Storage
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Alternative names
- 4 1BB
- 4 1BB ligand receptor
- 4-1BB ligand receptor
see all -
Function
Receptor for TNFSF14/4-1BBL. Possibly active during T cell activation. -
Tissue specificity
Expressed on the surface of activated T-cells. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
Images
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Functional Studies - Recombinant mouse CD137 protein (Fc Chimera) (ab220549)
Immobilized ab220549 at 2 μg/mL (100 μL/well) can bind Biotinylated Human 4‑1BB Ligand, Fc Tag with a linear range of 0.01-0.156 μg/mL.
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SDS-PAGE - Recombinant Mouse CD137 protein (Fc Chimera) (ab220549)SDS-PAGE analysis of ab220549 stained overnight with Coomassie Blue.
The reduced protein migrates as 50-66 kDa in SDS-PAGE.
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Functional Studies - Recombinant mouse CD137 protein (Fc Chimera) (ab220549)Loaded ab220549 on Protein A Biosensor, can bind Human 4-1BB Ligand (71-254), His, Flag Tag (active trimer) (MALS verified) with an affinity constant of 18.6 nM as determined in BLI assay (ForteBio Octet Red96e).
