Sucrose Assay Kit (ab83387)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.2 µM
Overview
-
Product name
Sucrose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
> 0.2 µM -
Range
0 µM - 10 µM -
Assay time
1h 00m -
Product overview
Abcam's Sucrose Assay Kit provides a convenient means for specifically measuring sucrose levels from various biological samples (e.g. serum, plasma, body fluids, food, growth medium, etc.). Sucrose is converted to glucose and fructose by invertase. The generated glucose is then specifically oxidized to generate a product which reacts with a probe to generate color (λ= 570 nm) and fluorescence (Ex/Em = 535/587 nm). Use of proper controls allows for correcting free glucose background. Other disaccharides such as lactose and maltose do not interfere with the assay. The method can detect 0.0002-10 mM sucrose concentrations.
Visit our FAQs page for tips and troubleshooting. -
Notes
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Enzyme Mix (lyophilized) Green 1 vial Invertase (Lyophilized) Blue1 1 vial OxiRed Probe in DMSO Red 1 x 200µl Sucrose Assay Buffer WM 1 x 25ml Sucrose Standard (Lyophilised) Yellow 1 x 100µl -
Research areas
-
Relevance
Sucrose (C12H22O11; FW:342.3), also called table sugar, is one of the most important fuel sources to generate the universal energy molecule ATP. Sucrose is a disaccharide which can be converted into one glucose and one fructose. -
Alternative names
- Saccharose
- Table sugar
Images
-
Functional Studies - Sucrose Assay Kit (ab83387)Standard Sucrose Assay. Different amounts of sucrose, lactose and maltose are assayed following the kit protocol. Data show that the Sucrose assay kit specifically detects sucrose, but not lactose or maltose.