Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Suitable for: WB, Functional Studies, SDS-PAGE
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Product name
Recombinant Influenza A Virus Hemagglutinin H7 protein -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Predicted molecular weight
62 kDa -
Additional sequence information
From virus strain A/Anhui/1/2013 (H7N9).
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Preparation and Storage
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Alternative names
- HA7
- Hemagglutinin 7
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Relevance
Haemagglutinin (HA) is one of two main surface fusion glycoproteins embedded in the envelope of influenza viruses, the other being neuraminidase (NA). There are sixteen known HA subtypes (H1-H16) and nine NA subtypes (N1-N9), which together are used to classify influenza viruses (e.g. H5N1). The antigenic variations in HA and NA enable the virus to evade host antibodies made to previous influenza strains, accounting for recurrent influenza epidemics.
Images
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Functional Studies - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)
Hemagglutination with turkey red blood cells: Hemagglutinin Titer 1:2048
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SDS-PAGE - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)SDS-PAGE analysis of 0.4 µg ab190421 under reducing conditions.
The theoretical molecular weight of the protein is ~62 kDa, without glycosylation. Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
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Western blot - Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421)Anti-H7 NY mouse antiserum at 1/100 dilution +Recombinant Influenza A Virus Hemagglutinin H7 protein (ab190421) at 0.38 µg
Secondary
Anti-mouse AP conjugateQuality control testing demonstrates strong detection of GP null under reduced conditions when using anti-H7 influenza antiserum.