Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant human WISP1 protein (ab50041)

Key features and details

  • Expression system: Escherichia coli
  • Endotoxin level:
  • Active: Yes
  • Suitable for: SDS-PAGE, Functional Studies

Description

  • Product name

    Recombinant human WISP1 protein
    See all WISP1 proteins and peptides

  • Biological activity

    Biological Activity : The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 µg/ml.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Protein length

    Full length protein

  • Animal free

    No

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN

    • Associated products

      Specifications

      Our Abpromise guarantee covers the use of ab50041 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      • Applications

        SDS-PAGE

        Functional Studies

      • Form

        Lyophilized
      • Concentration information loading...

      Preparation and Storage

      • Stability and Storage

        Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.

        Constituent: 0.06% Acetic acid

        This product is an active protein and may elicit a biological response in vivo, handle with caution.

      • Reconstitution
        Reconstitute product with 10mM Acetic Acid.

      General Info

      • Alternative names

        • CCN 4
        • CCN family member 4
        • CCN4
        • WISP 1
        • WISP 1c
        • WISP 1i
        • WISP 1tc
        • WISP1c
        • WISP1i
        • WISP1tc
        • Wnt 1 induced secreted protein
        • WNT 1 induced secreted protein 1
        • WNT 1 inducible signaling pathway protein 1
        • Wnt 1 signaling pathway protein 1
        • Wnt-1 inducible signaling pathway protein 1
        • Wnt1 induced secreted protein
        • WNT1 induced secreted protein 1
        • WNT1 inducible signaling pathway protein 1
        • Wnt1 signaling pathway protein 1
        • WNT1-inducible-signaling pathway protein 1
        see all

      • Relevance

        WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.

      • Cellular localization

        Secreted

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab50041? Please let us know so that we can cite the reference in this datasheet.

    ab50041 has been referenced in 3 publications.

    • Rong X  et al. Human bone marrow mesenchymal stem cells-derived exosomes alleviate liver fibrosis through the Wnt/ß-catenin pathway. Stem Cell Res Ther 10:98 (2019). PubMed: 30885249
    • Meyers CA  et al. WISP-1 drives bone formation at the expense of fat formation in human perivascular stem cells. Sci Rep 8:15618 (2018). PubMed: 30353078
    • Li WF  et al. WISP-1 contributes to fractionated irradiation-induced radioresistance in esophageal carcinoma cell lines and mice. PLoS One 9:e94751 (2014). ICC/IF ; Mouse, Human . PubMed: 24728101

    Preparation and Storage

    • Alternative names

      • CCN 4
      • CCN family member 4
      • CCN4
      • WISP 1
      • WISP 1c
      • WISP 1i
      • WISP 1tc
      • WISP1c
      • WISP1i
      • WISP1tc
      • Wnt 1 induced secreted protein
      • WNT 1 induced secreted protein 1
      • WNT 1 inducible signaling pathway protein 1
      • Wnt 1 signaling pathway protein 1
      • Wnt-1 inducible signaling pathway protein 1
      • Wnt1 induced secreted protein
      • WNT1 induced secreted protein 1
      • WNT1 inducible signaling pathway protein 1
      • Wnt1 signaling pathway protein 1
      • WNT1-inducible-signaling pathway protein 1
      see all

    • Relevance

      WISP1 is a downstream regulator in the Wnt/Frizzled-signaling pathway and is associated with cell survival. WISP1 attentuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase and up-regulates the anti-apoptotic Bcl-X(L) protein. WISP1 is expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Overexpression is associated with several cancers including breast cancer and colon tumors.

    • Cellular localization

      Secreted

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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