Recombinant human USP10 protein (ab198669)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 60% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human USP10 protein -
Biological activity
Specific Activity: 23 pmol/min/µg.
Assay Conditions:
Reaction was performed in 50 mM Tris, pH 7.4, 1 mM DTT, 0.5 mM EDTA, 500 nM Ub-AMC, and ab198669 in a 50 µL volume. Reaction was incubated at 37°C for 15 min. and fluorescent signal was measured at excitation = 340 nm, emission = 460 nm. -
Purity
>= 60 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
ALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAVDKLPDG QEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITP DGITKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKK KKKRPPGYYSYLKDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMP PSVTPRTCNSPQNSTDSVSDIVPDSPFPGALGSDTRTAGQPEGGPGADFG QSCFPAEAGRDTLSRTAGAQPCVGTDTTENLGVANGQILESSGEGTATNG VELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPKSWASLFHDSKP SSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAELLE NVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSK VQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEP TYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNE KLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTP ITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLV ARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLI KNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYT TDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL -
Predicted molecular weight
88 kDa including tags -
Amino acids
2 to 798 -
Tags
DDDDK tag N-Terminus
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Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Contains 90 µg/mL DDDDK peptideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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Functional Studies - Recombinant human USP10 protein (ab198669)
Example of specific activity of ab198669.
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SDS-PAGE - Recombinant human USP10 protein (ab198669)10% SDS-PAGE with Coomassie staining: 3 µg ab198669.
