Anti-UCHL3 antibody (ab241490)
Key features and details
- Rabbit polyclonal to UCHL3
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-UCHL3 antibody
See all UCHL3 primary antibodies -
Description
Rabbit polyclonal to UCHL3 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig
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Immunogen
Synthetic peptide within Human UCHL3 aa 174-224. The exact sequence is proprietary. (NP_005993.1).
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Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate. IHC-P: Human testicular seminoma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241490 was affinity purified using an epitope specific to UCHL3 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-UCHL3 antibody (ab241490)All lanes : Anti-UCHL3 antibody (ab241490) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 26 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-UCHL3 antibody (ab241490)UCHL3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab241490 at 3 µg/mg lysate. Western blot was performed on the immunoprecipitate using ab241490 at 1 µg/ml.
Lane 1: ab241490 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.Detection: Chemiluminescence at an exposure time of 3 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCHL3 antibody (ab241490)Formalin-fixed, paraffin-embedded human testicular seminoma tissue stained for UCHL3 using ab241490 at 1/200 dilution in immunohistochemical analysis. DAB staining.
