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Recombinant human TWEAK protein (Animal Free) (ab217458)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 98% SDS-PAGE
  • Active: Yes
  • Suitable for: Functional Studies, SDS-PAGE, HPLC

Description

  • Product name

    Recombinant human TWEAK protein (Animal Free)
    See all TWEAK proteins and peptides

  • Biological activity

    Assay #1:  The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 10 ng/ml. 
    Assay #2:  TWEAK weakly induces the death of HT29 cells when cultured in the presence of IFN-γ.  The ED50 for this effect is between 30-45 ng/ml.

  • Purity

    > 98 % SDS-PAGE.
    assessed also by HPLC

  • Expression system

    Escherichia coli

  • Accession

    O43508

  • Protein length

    Protein fragment

  • Animal free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MKGRKTRARR AIAAHYEVHP RPGQDGAQAG VDGTVSGWEEARINSSSPLRYNRQIGEFIVTRAGLYYLYCQVHFDEGKAV YLKLDLLVDGVLALRCLEEFSATAASSLGPQLRLCQVSGLLALRPGSSLR IRTLPWAHLKAAPFLTYFGLFQVH

    • Predicted molecular weight

      17 kDa

    • Amino acids

      97 to 249

    • Additional sequence information

      comprising the TNF-homologous region of TWEAK, generated by proteolytic processing of the full length membrane-anchored TWEAK protein

Preparation and Storage

  • Alternative names

    • APO 3 ligand
    • APO 3L
    • APO3 ligand
    • APO3/DR3 ligand
    • APO3L
    • DR3LG
    • MGC129581
    • MGC20669
    • secreted form
    • TNF-related weak inducer of apoptosis
    • TNF12_HUMAN
    • TNFSF 12
    • Tnfsf12
    • TNFSF12 protein
    • Tumor necrosis factor (ligand) superfamily member 12
    • Tumor necrosis factor ligand superfamily member 12
    • Tumor necrosis factor superfamily member 12
    • TWEAK
    • UNQ181/PRO207
    see all

  • Function

    Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines.

  • Tissue specificity

    Highly expressed in adult heart, pancreas, skeletal muscle, brain, colon, small intestine, lung, ovary, prostate, spleen, lymph node, appendix and peripheral blood lymphocytes. Low expression in kidney, testis, liver, placenta, thymus and bone marrow. Also detected in fetal kidney, liver, lung and brain.

  • Sequence similarities

    Belongs to the tumor necrosis factor family.

  • Post-translational
    modifications

    The soluble form derives from the membrane form by proteolytic processing.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

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