Recombinant human TNFRSF14/HVEM protein (ab155629)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.4
Constituents: Tris, Glycine, L-Arginine, Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 400 µg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human TNFRSF14/HVEM protein (ab155629)Human HVEM, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human TNFRSF14/HVEM protein (ab155629)Immobilized Human HVEM, Fc Tag at 5 µg/mL (100 µL/well) can bind Biotinylated Human BTLA (31-134), Fc,Avitag with a linear range of 0.156-5 µg/mL. -
Functional Studies - Recombinant human TNFRSF14/HVEM protein (ab155629)Immobilized Human LIGHT, Mouse IgG2a Fc Tag, low endotoxin at 2 µg/mL (100 µL/well) can bind Human HVEM, Fc Tag with a linear range of 0.3-20 ng/mL. -
SDS-PAGE - Recombinant human TNFRSF14/HVEM protein (ab155629)SDS-PAGE of reduced ab155629 stained overnight with Coomassie Blue.
