Recombinant human TIMP2 protein (ab82117)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant human TIMP2 protein
See all TIMP2 proteins and peptides -
Biological activity
TIMP2 activity was measured by its ability to inhibit human MMP1 induced hydrolysis of a chromogenic peptide substrate at room temperature. Half maximal inhibition was obtained at a TIMP2 concentration of approximately 0.5 µg/ml, when using an MMP1 concentration of 1.6 µg/ml.
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Purity
> 95 % SDS-PAGE.
Purity is greater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
CSCSPVHPQ QAFCNADVVI RAKAVSEKEV DSGNDIYGNP IKRIQYEIKQ IKMFKGPEKD IEFIYTAPSS AVCGVSLDVG GKKEYLIAGK AEGDGKMHIT LCDFIVPWDT LSTTQKKSLN HRYQMGCECK ITRCPMIPCY ISSPDECLWM DWVTEKNING HQAKFFACIK RSDGSCAWYR GAAPPKQEFL DIEDP -
Amino acids
2 to 220
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionCentrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex (can be stored at 4C for up to 1 week). For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20C to -80C.
