Recombinant human TGF alpha protein (Animal Free) (ab217389)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies, HPLC
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Product name
Recombinant human TGF alpha protein (Animal Free)
See all TGF alpha proteins and peptides -
Biological activity
Determined by its ability to stimulate the proliferation of mouse Balb/c 3T3 cells. The expected ED50 is ≤ 0.2 ng/mL, corresponding to a specific activity of ≥ 5 x 106 units/mg.
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Purity
> 98 % SDS-PAGE.
assessed also by HPLC -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
Yes -
Nature
Recombinant -
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Species
Human -
Sequence
VVSHFNDCPD SHTQFCFHGT CRFLVQEDKP ACVCHSGYVG ARCEHADLLA -
Predicted molecular weight
6 kDa -
Amino acids
40 to 89 -
Additional sequence information
Full length mature chain including 6 conserved cysteine residues, which form 3 intramolecular disulfide bonds. This sequence shares approximately 40% sequence homology with EGF.
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Preparation and Storage
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Alternative names
- EGF like TGF
- EGF-like TGF
- ETGF
see all -
Function
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar. -
Tissue specificity
Isoform 1, isoform 3 and isoform 4 are expressed in keratinocytes and tumor-derived cell lines. -
Sequence similarities
Contains 1 EGF-like domain. -
Cellular localization
Cell membrane and Secreted > extracellular space. - Information by UniProt
