Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
![Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)](https://www.abcam.com/ps/products/229/ab229820/Images/ab229820-1-229820ELISA1.jpg "Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3
- Suitable for: Competitive ELISA
- Reacts with: Human
Overview
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Product name
Anti-Vitamin D3 antibody [EPPTX-R6-42-12]
See all Vitamin D3 primary antibodies -
Description
Rabbit monoclonal [EPPTX-R6-42-12] to Vitamin D3 -
Host species
Rabbit -
Tested applications
Suitable for: Competitive ELISAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Chemical/ Small Molecule. Vitamin-D-3-BSA.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPPTX-R6-42-12 -
Isotype
IgG -
Research areas
Images
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Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
Goat anti-rabbit IgG was coated onto 96-wells. Serial dilutions (0, 2.5, 5, 10, 25, 50, 100 ng/mL) of 25-OH-Vitamin-D (25 μL) were incubated with 20-25 ng/mL of ab229820 (50 μL) for 30 minutes by shaking.
This was followed by adding HRP-conjugated 25-OH-Vitamin-D (50 μL) into each well and incubating for another 30 minutes. After washing, 125 μL of Tetramethylbenzidine (TMB) substrate was added and incubated for 30 minutes without shaking for color development. OD was read at 450nm within 5 minutes.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)](https://www.abcam.com/ps/products/229/ab229820/Images/ab229820-2-229820ELISA2.jpg)
Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)Competitive ELISA - Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820) used at 6.3 ng/ml.
This data was kindly provided by Pantex Division of Bio-Analysis, Inc.
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![Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)](https://www.abcam.com/ps/products/229/ab229820/Images/ab229820-3-benefits-of-recombinant-antibodies.png)
Anti-Vitamin D3 antibody [EPPTX-R6-42-12] (ab229820)
