Recombinant human SynCAM/CADM1 protein (ab167740)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 94% PBS, 5% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 100 ug/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.
Images
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SDS-PAGE - Recombinant human SynCAM/CADM1 protein (ab167740)Human CADM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Functional Studies - Recombinant human SynCAM/CADM1 protein (ab167740)Immobilized Cynomolgus CRTAM, Fc Tag at 5 µg/mL (100 µL/well) can bind Human CADM1, His Tag with a linear range of 0.6-5 ng/mL. -
SDS-PAGE - Recombinant human SynCAM/CADM1 protein (ab167740)SDS-PAGE analysis of reduced ab167740 and staining overnight with Coomassie Blue. In DTT-reduced SDS-PAGE, SynCAM/CADM1 protein migrates as 38.5kDa, 50kDa and 55-85 kDa poly peptide due to different glycosylation.