Recombinant Human Serum Albumin protein (His tag) (ab217817)
Key features and details
- Expression system: HEK 293 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level:
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
pH: 7.40
Constituents: 90% PBS, 10% Trehalose
Lyophilised from 0.22 µm filtered solution. -
ReconstitutionReconstitute with sterile deionized water to a concentration of 200 µg/ml.
Images
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SDS-PAGE analysis of DTT-reduced ab217817 followed by staining overnight with Coomassie Blue.
DTT-reduced protein migrates as 66 kDa in SDS-PAGE due to glycosylation.
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Bioactivity SPR: Immobilized biotinyated Huamn FcRN Heterodimer protein on SA chip can bind ab217817, his tag with an affitinity constant of 1.78μM.