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Neuroscience Neurology process Growth and Development Axonal Guidance Proteins

Recombinant Human SEMAC protein (ab191921)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, HPLC

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Description

  • Product name

    Recombinant Human SEMAC protein
  • Purity

    > 95 % SDS-PAGE.
    Purity greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
  • Endotoxin level

  • Expression system

    HEK 293 cells
  • Accession

    Q9NPR2
  • Protein length

    Protein fragment
  • Animal free

    No
  • Nature

    Recombinant
    • Species

      Human
    • Sequence

      LSPRISLPLGSEERPFLRFEAEHISNYTALLLSRDGRTLYVGAREALFAL SSNLSFLPGGEYQELLWGADAEKKQQCSFKGKDPQRDCQNYIKILLPLSG SHLFTCGTAAFSPMCTYINMENFTLARDEKGNVLLEDGKGRCPFDPNFKS TALVVDGELYTGTVSSFQGNDPAISRSQSLRPTKTESSLNWLQDPAFVAS AYIPESLGSLQGDDDKIYFFFSETGQEFEFFENTIVSRIARICKGDEGGE RVLQQRWTSFLKAQLLCSRPDDGFPFNVLQDVFTLSPSPQDWRDTLFYGV FTSQWHRGTTEGSAVCVFTMKDVQRVFSGLYKEVNRETQQWYTVTHPVPT PRPGACITNSARERKINSSLQLPDRVLNFLKDHFLMDGQVRSRMLLLQPQ ARYQRVAVHRVPGLHHTYDVLFLGTGDGRLHKAVSVGPRVHIIEELQIFS SGQPVQNLLLDTHRGLLYAASHSGVVQVPMANCSLYRSCGDCLLARDPYC AWSGSSCKHVSLYQPQLATRPWIQDIEGASAKDLCSASSVVSPSFVPTGE KPCEQVQFQPNTVNTLACPLLSNLATRLWLRNGAPVNASASCHVLPTGDL LLVGTQQLGEFQCWSLEEGFQQLVASYCPEVVEDGVADQTDEGGSVPVII STSRVSAPAGGKASWGADRSYWKEVDDIEGRMDEPKSCDKTHTCPPCPAP ELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGV EVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPI EKTISKAKGQPREPQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWE SNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEAL HNHYTQKSLSLSPGKHHHHHH
    • Predicted molecular weight

      103 kDa including tags
    • Amino acids

      39 to 712
    • Tags

      His tag C-Terminus
    • Additional sequence information

      Extracellular domain fused with a polyhistidine tag and FC tag at the C-terminus.
  • Associated products

    • Related Products

      • Anti-6X His tag® antibody [HIS.H8] (ab18184)
      • Anti-6X His tag® antibody [4D11] (ab5000)
      • Anti-6X His tag® antibody (ab9108)

    Specifications

    Our Abpromise guarantee covers the use of ab191921 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Lyophilized
    • Additional notes

       This product was previously labelled as SEMA4B

       

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      Lyophilized from a 0.2 µM filtered solution.

    • Reconstitution
      Dissolve protein in 1 x PBS to a concentration no less than 100ug/ml.

    General Info

    • Alternative names

      • KIAA1745
      • MGC131831
      • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain (semaphorin) 4B
      • Sema domain immunoglobulin domain (Ig) transmembrane domain (TM) and short cytoplasmic domain 4B
      • SEMA4B
      • SEMAC
      • Semaphorin 4B
      • SemC
      see all
    • Relevance

      SEMA4B belongs to the semaphorin family and contains 1 Ig-like C2-type (immunoglobulin-like) domain, 1 PSI domain and 1 Sema domain. It inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.
    • Cellular localization

      Membrane

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab191921? Please let us know so that we can cite the reference in this datasheet.

    ab191921 has not yet been referenced specifically in any publications.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituent: 100% PBS

      Lyophilized from a 0.2 µM filtered solution.

    • Reconstitution
      Dissolve protein in 1 x PBS to a concentration no less than 100ug/ml.

    Images

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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